LOCUS       sequence-59436-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Su PC, Si W, Baker DL, Berger BW
  TITLE     High-Yield membraneprotein expression fromE. coliusing an engineered
            outer membrane protein F (OmpF) fusion.
  JOURNAL   Protein Sci. 2013 Jan 24. doi: 10.1002/pro.2224.
  PUBMED    23345122
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2013 Jan 24. doi: 10.1002/pro.2224."
COMMENT     Sequence Label: pOmpF
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(114..131)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(573..590)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             609..631
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    646..670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(670..689)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(671..689)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(773..792)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tctttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcg gatccgctag cactagtggt accgctgccg
      121 cgcggcacca ggccgccgcc gccgccggag ttacgatagg tagcaacccc gccaacacga
      181 ccaacgaaga agtcatcgct gtatgcagta tcaccaccaa attctggcag catatcggtg
      241 taacccagtg catcataaac cacaccgtag ttacggccgt aatcgaaaga accaacgtca
      301 gcgtatttaa gacccgcgaa tgccagacgc gttttgttac cagtttgagc gtcagcgcct
      361 tcagagttgt taccctggaa gttatattcc cactgaccat aaccggtcag atcggaattg
      421 atttgagttt cccctttaaa accaagacgg gcataggtca tgtcgccatt gccaccgtaa
      481 ctgttttcac cgttaccctt ggaaaaataa tgcagaccaa cagctttacc gtacagatct
      541 actttgttgc catctttgtt atagatttct gcgtgatgat gatgatgatg gctgctgccc
      601 atggtatatc tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      661 tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      721 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      781 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      841 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      901 gcaccattcc ttgcggcggc gggctcaacg g
//
