LOCUS       sequence-59446-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Lukovic E, Vogel Taylor E, Imperiali B
  TITLE     Monitoring protein kinases in cellular media with highly selective 
            chimeric reporters.
  JOURNAL   Angew Chem Int Ed Engl. 2009;48(37):6828-31. doi: 
            10.1002/anie.200902374.
  PUBMED    19681083
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl."; date: "2009"; volume: "48(37):6828-31. doi"; pages: "
            10.1002/anie.200902374"
COMMENT     Sequence Label: GST-ENLYFQC-PNT(46-138)-His6
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..73)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(389..406)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(433..455)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gtcatcaccg aaacgcgcga ggcagatcgt cagtcagtca cgatgcggcc gcctagtgat
       61 ggtgatggtg atgacctttc acatcctctt tctgcaggat ctctagatgc tcccacagga
      121 tatccccaac aaagtctgga gccagctcga ggaagcattc tttacccagg gcacacagtg
      181 ctgctccact catgcagaac ttctggaagt ccacaccttt caggctgaac tcattcacag
      241 cccacatcac ccagtcccgg acgtgggttt ctgtccactg ccgggggtct ttggggattc
      301 ccagtcgctg ctgttctttt gtgaaaccac tgaaagtagc tttcaaggct tgggagcact
      361 gaaaatacag gttttcacga attcctgggg atccacgcgg aaccagatcc gattttggag
      421 gatggtcgcc accaccaaac gtggcttgcc agccctgcaa aggccatgct atatacttgc
      481 tggatttcaa gtacttatca atttgtggga tagcttcaat acgtttttta aaacaaacta
      541 attttgggaa cgcatccagg cacattgggt ccatgtataa aacaacatca agagcgtcat
      601 acaacatgaa gtcaggatgg gttacatgat caccatttaa atatgtttta tgacataaac
      661 gatcttcgaa cattttcagc atttcaggta gcttgctaag aaaatcaact ttgagagttt
      721 caaagtcttt actatatgca attctcgaaa caccgtatct aatatccaaa accgctcctt
      781 caagcattga aatctctgca cgctcttttg gacaaccacc caacatgttg tgcttgtcag
      841 ctatataacg tatgatggcc atagactgtg ttaatttaac atcaccatca atataataag
      901 gaagattggg aaactccaaa cccaattcaa actttttgtt tcgccattta tcaccttcat
      961 cgcgctcata caaatgctct tcatattttt cttcaagata ttccaaaaga agtcga
//
