LOCUS       sequence-59448-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Harada H, Becknell B, Wilm M, Mann M, Huang LJ, Taylor SS, Scott JD,
            Korsmeyer SJ
  TITLE     Phosphorylation and inactivation of BAD by mitochondria-anchored 
            protein kinase A.
  JOURNAL   Mol Cell 1999 Apr;3(4):413-22.
  PUBMED    10230394
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "1999-04"; volume: "3(4)"; pages: "413-22"
COMMENT     Sequence Label: 3604 pGEX4T-1 Bad S136A (104-141)
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(190..207)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(214..867)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(234..256)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(879..901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    890..906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(914..920,921..936,937..942))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gtcatcaccg aaacgcgcga ggcagatcgt cagtcagtca cgatgcggcc gctcgagtcg
       61 acccgggaat tctcagagat tggggggagc cgcacgcgag cgtcctcgaa aagggctaag
      121 ctcctcctcc atcccttcat cctcctcggt ccccgctggg tacgaactgt ggcgactccg
      181 agtctccatg gatccacgcg gaaccagatc cgattttgga ggatggtcgc caccaccaaa
      241 cgtggcttgc cagccctgca aaggccatgc tatatacttg ctggatttca agtacttatc
      301 aatttgtggg atagcttcaa tacgtttttt aaaacaaact aattttggga acgcatccag
      361 gcacattggg tccatgtata aaacaacatc aagagcgtca tacaacatga agtcaggatg
      421 ggttacatga tcaccattta aatatgtttt atgacataaa cgatcttcga acattttcag
      481 catttcaggt agcttgctaa gaaaatcaac tttgagagtt tcaaagtctt tactatatgc
      541 aattctcgaa acaccgtatc taatatccaa aaccgctcct tcaagcattg aaatctctgc
      601 acgctctttt ggacaaccac ccaacatgtt gtgcttgtca gctatataac gtatgatggc
      661 catagactgt gttaatttaa catcaccatc aatataataa ggaagattgg gaaactccaa
      721 acccaattca aactttttgt ttcgccattt atcaccttca tcgcgctcat acaaatgctc
      781 ttcatatttt tcttcaagat attccaaaag aagtcgagtg ggttgcacaa ggcccttaat
      841 tttccaataa cctagtatag gggacatgaa tactgtttcc tgtgtgaaat tgttatccgc
      901 tcacaattcc acacattata cgagccgatg attaattgtc aacagctcat ttcaga
//
