LOCUS       sequence-59450-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     Landry Lab Human Histone Expression Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Pet28a_Human_H2B.1
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             454..476
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    491..515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(515..534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(516..534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(618..637)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcttattac ttggagcttg tatacttggt gacggccttg gtgccctcgg acacggcgtg
      121 cttggccaat tccccgggta gcagtaggcg cacggccgtc tggatctccc tcgaagtgat
      181 ggtcgagcgc ttgttgtaat gcgccaggcg tgacgcttct ccggcgatac gctcaaagat
      241 gtcgttgacg aaggagttca tgattcccat agccttggaa gagatgccgg tgtcggggtg
      301 gacctgcttc agcaccttgt acacatacac agagtagctc tccttgcggc tgcgtttgcg
      361 cttctttcca tccttcttct gcgccttggt cacagccttc ttggaaccct tcttcggagc
      421 aggagctgac ttagctggtt caggcatggt atatctcctt cttaaagtta aacaaaatta
      481 tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
      541 cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      601 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      661 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      721 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      781 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc
      841 cggacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
      901 gtcaattcag ggtggtga
//
