LOCUS       sequence-59462-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-gw
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    125..249
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(290..595)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(392..411)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtttaaatag tacataatgg atttccttac gcgaaatacg ggcagacatg gcctgcccgg
       61 ttattattat ttttgacacc agaccaactg gtaatggtag cgaccggcgc tcagctggaa
      121 tatcaccact ttgtacaaga aagctgaacg agaaacgtaa aatgatataa atatcaatat
      181 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
      241 gtcactatgg tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc
      301 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
      361 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
      421 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
      481 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
      541 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
      601 cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
      661 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
      721 atggttgtgc ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc
      781 tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact
      841 tcg
//
