LOCUS       sequence-59463-e        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F
  TITLE     Comprehensive interrogation of natural TALE DNA-binding modules and 
            transcriptional repressor domains.
  JOURNAL   Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
  PUBMED    22828628
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-07-24"; volume: "3:968. doi"; pages: " 
            10.1038/ncomms1962"
COMMENT     Sequence Label: SOX2 TALE Repressor (KRAB 11-75)
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..77)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(93..287)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(297..317)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacagctcct cgcccttgct cactgggcca ggattctcct cgacgtcacc gcatgttagc
       61 agacttcctc tgccctctcc actgcctcta gataccagcc aaggttcttc ccccttttcg
      121 agcctaagga tcacatccgg tttcgtcaac tggtatccga gactgacgag gttcttgtaa
      181 ttctcaagca tgacatttcg atacacaatt tgttgcgcag tatccagcaa tttccactcc
      241 tcacgtgtaa agtccacaaa cacgtccttg aacgtaacca gtgttctgct ggcctccacc
      301 tttctcttct tcttggggct agctgacgcg cgagtttggt ccccttcatg catgggcgag
      361 ggcgcgtcca aatccctctc cagcgagtct gcgaacgcat gaaggctcgc ctggtcaggt
      421 gtctgagttg acgtaggtga aggcttcgcg cgtttcatac cgctcgcttg gagaatcctg
      481 tcccaccgct gggaggcggg aggcagtgtg cccgagcggg cctcgagttc tgtgacaccg
      541 actctacgaa agagctgcag cagtccgtgt ctcgacatac caaattgagt catggcgtca
      601 tcgaacgctt gtgcggggtg ggagtgacac tggaagaatc cgagcacgcg gaccacttgc
      661 gcgtgatctg ccactcgatg tgatgtcctc tcgggaatcc ttctgttggt ccgcttaatc
      721 aatgcaggcg cgtgcgggag ccccttcttc accgcatcca aggcgggtcg tccgccgagg
      781 caggccagcg ctacaagatg atcattagtg agtgcggcca gcgcggggtc cggcctcgaa
      841 agctgggcca cgattgactc cagtgcgggt ctgcccccga tattggatgc aatagccact
      901 acctgctcag gcgtgagtcc gtgcgcttgg cacagcacag gcaacagcct ctgtactgtt
      961 tccaaggctt gctttcctcc gtcgtggctt gcgatcgcca cgacctgctc tggggttaac
     1021 ccgtgcgctt ggcaca
//
