LOCUS       sequence-59464-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  TITLE     Lok lab Strongyloides stercoralis plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPV475
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..177)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(779..874)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(911..930)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(912..930)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(935..951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(935..951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cagctctaga gctcgaattc tacaggtcac taataccatc taagtagttg gttcatagtg
       61 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      121 atatattgat atttatatca ttttacgttt ctcgttcaac tttcttgtac aaagtggccc
      181 taggaattta ttttgataag tctcgcgtat tttatttgta ataatctttt tataagatca
      241 atttacattt atactgataa aattgacaca attacacttt ttattataaa atattttaaa
      301 atatttctat caaaaggttt atttttgttt taaatataat ggtttttttt gtaaaaaata
      361 cttatttcgt ttgataatct ttattcagag atttgaataa ttttaaataa agtagtttag
      421 ttttcatata aaagttaatg gtatatataa atatttaaat agtaaagatt gaatcagtat
      481 tcaaaatgac acctaaaagt tattctattt ctagctataa caataataat aaagataatt
      541 atgatacaga tttagaaaaa aaaataaggt taaattaatt aattctattt ttaattaaaa
      601 tgatttaagg gagttaaatg aaacagaaac agattgggtc tctatatatt tagtttatgt
      661 aatgttattt tttggtggaa tacaaataac ggtatatttt acttcaatgt ggccatactt
      721 atcaacaata aatagtaagg caaatgtaga tactttaggg tgggttgtag cactagtgca
      781 actttattat acaaagttgg cattataaaa aagcattgct tatcaatttg ttgcaacgaa
      841 caggtcacta tcagtcaaaa taaaatcatt atttggagct ccatggtagc gttaacgcgg
      901 ccgcgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
      961 gcccgtgtct caaaatctct ga
//
