LOCUS       sequence-59465-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F
  TITLE     Comprehensive interrogation of natural TALE DNA-binding modules and 
            transcriptional repressor domains.
  JOURNAL   Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
  PUBMED    22828628
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-07-24"; volume: "3:968. doi"; pages: " 
            10.1038/ncomms1962"
COMMENT     Sequence Label: SOX2 TALE Repressor (KRAB 1-75)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..77)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(93..287)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(327..347)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacagctcct cgcccttgct cactgggcca ggattctcct cgacgtcacc gcatgttagc
       61 agacttcctc tgccctctcc actgcctcta gataccagcc aaggttcttc ccccttttcg
      121 agcctaagga tcacatccgg tttcgtcaac tggtatccga gactgacgag gttcttgtaa
      181 ttctcaagca tgacatttcg atacacaatt tgttgcgcag tatccagcaa tttccactcc
      241 tcacgtgtaa agtccacaaa cacgtccttg aacgtaacca gtgttctcga ccatgccgta
      301 agtgatttcg cgtccatgct ggcctccacc tttctcttct tcttggggct agctgacgcg
      361 cgagtttggt ccccttcatg catgggcgag ggcgcgtcca aatccctctc cagcgagtct
      421 gcgaacgcat gaaggctcgc ctggtcaggt gtctgagttg acgtaggtga aggcttcgcg
      481 cgtttcatac cgctcgcttg gagaatcctg tcccaccgct gggaggcggg aggcagtgtg
      541 cccgagcggg cctcgagttc tgtgacaccg actctacgaa agagctgcag cagtccgtgt
      601 ctcgacatac caaattgagt catggcgtca tcgaacgctt gtgcggggtg ggagtgacac
      661 tggaagaatc cgagcacgcg gaccacttgc gcgtgatctg ccactcgatg tgatgtcctc
      721 tcgggaatcc ttctgttggt ccgcttaatc aatgcaggcg cgtgcgggag ccccttcttc
      781 accgcatcca aggcgggtcg tccgccgagg caggccagcg ctacaagatg atcattagtg
      841 agtgcggcca gcgcggggtc cggcctcgaa
//
