LOCUS       sequence-59510-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  TITLE     Lok lab Strongyloides stercoralis plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPV476
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..174)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(207..235)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctctagagct cgaattctac aggtcactaa taccatctaa gtagttggtt catagtgact
       61 gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      121 tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa gtggccatga
      181 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      241 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      301 ttacccttaa atttatttgc actactggaa aactacctgt tccatgggta agtttaaaca
      361 tatatatact aactaaccct gattatttaa attttcagcc aacacttgtc actactctca
      421 cttatggtgt tcaatgcttc tcgagatacc cagatcatat gaaacagcat gactttttca
      481 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      541 actacaagac acgtaagttt aaacagttcg gtactaacta accatacata tttaaatttt
      601 caggtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      661 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      721 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttgta agtttaaaca
      781 tgattttact aactaactaa tctgatttaa attttcagaa cttcaaaatt agacacaaca
      841 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      901 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
      961 ccaacgaaaa gagagaccac atggtccttc ttgagtt
//
