LOCUS       sequence-59511-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  TITLE     Lok lab Strongyloides stercoralis plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPV477
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(139..167)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     915..935
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(980..1004)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 gagcaatgct tttttataat gccaactttg tacaaaaaag caggctccat gagtaaagga
      121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      241 aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa catatatata
      301 ctaactaacc ctgattattt aaattttcag ccaacacttg tcactactct cacttatggt
      361 gttcaatgct tctcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      421 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      481 acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct
      541 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      601 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      661 tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta
      721 ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa cattgaagat
      781 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      841 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      901 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      961 gacgaactat acaaatgaga cccagctttc ttgtacaaag ttggcattat a
//
