LOCUS       sequence-59514-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  TITLE     Landry Lab Human Histone Expression Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Pet28a_Human_H3.1
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             485..507
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    522..546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(546..565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(547..565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(649..668)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        874..951
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgccttatca cgccctctct ccgcggatgc ggcgcgcaag ctggatgtct ttaggcataa
      121 tagtgacgcg tttggcgtga atggcgcaca ggttggtatc ctcaaatagc cccaccaggt
      181 aggcctcgca agcctcctgc agcgccatca ccgccgagct ctggaagcgc agatcggtct
      241 taaagtcctg tgcgatctcc cgtaccaagc gctgaaaagg tagcttccgg attagcagct
      301 cggtcgactt ctggtagcgg cggatctcgc gcagggccac ggtgccgggg cggtagcggt
      361 ggggcttctt gacgccaccg gtggccggag cgctcttgcg agccgccttg gtggccagct
      421 gcttgcgcgg cgctttgccg ccggtggact tgcgagcagt ttgcttagtg cgagccatgg
      481 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      541 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
      601 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      661 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      721 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      781 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      841 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt
      901 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
      961 gtaacgttat acgatgtcgc aga
//
