LOCUS       sequence-59515-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  TITLE     Landry Lab Human Histone Expression Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Pet28a_Human_H3.3
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             489..511
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    526..550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(550..569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(551..569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(653..672)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        878..955
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(975..994)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcctt attaagcacg ttctccacgt atgcggcgtg ctagctggat gtcttttggc
      121 ataattgtta cacgtttggc atggatagca cacaggttgg tgtcttcaaa aaggccaacc
      181 agataggcct cacttgcctc ctgcaaagca ccgatagctg cgctctggaa gcgcagatct
      241 gttttaaagt cctgagcaat ttctcgcacc agacgctgga agggaagttt gcgaatcaga
      301 agttcagtgg acttctgata acgtctaatt tcacggagcg ccacagtacc aggcctgtaa
      361 cgatgaggtt tcttcacccc tccagtagag ggcgcactct tgcgagcggc ttttgtagcc
      421 agttgcttcc tgggtgcttt accaccggtc gatttgcggg cagtctgctt tgtacgagcc
      481 atggtatatc tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      541 tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      601 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      661 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      721 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      781 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      841 atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac
      901 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
      961 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
     1021 cgtggtgaac caggccagcc acgttt
//
