LOCUS       sequence-59516-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  TITLE     Landry Lab Human Histone Expression Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Pet28a_Human_H4
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             390..412
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    427..451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(451..470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(452..470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(554..573)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        779..856
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(876..895)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcctt actagcctcc gaagccgtac agggtgcgcc cctggcgctt gagcgcgtac
      121 accacatcca tggctgtgac ggtcttgcgc ttggcgtgct cggtgtaggt gactgcgtcc
      181 cgaatcacat tctccaggaa caccttcagc acaccgcggg tctcctcgta aatgaggcca
      241 gagatccgct taacgccgcc acgccgagct agacgccgaa tggcaggctt ggtgatgccc
      301 tgaatgttgt ctctcaagac cttgcggtgg cgcttagcgc cccctttgcc taagcctttt
      361 ccgccctttc ctctgccgga catggtatat ctccttctta aagttaaaca aaattatttc
      421 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg
      481 atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      541 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      601 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      661 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      721 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga
      781 caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
      841 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
      901 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
      961 ggaaaaa
//
