LOCUS       sequence-59517-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  TITLE     Landry Lab Human Histone Expression Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Pet28a_Synthetic_Human_H2A.1
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             470..492
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    507..531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(531..550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(532..550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(634..653)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        859..936
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(956..975)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgctca tcatttgcct ttcgctttgt ggtgagattc ggtttttttc ggcagcagaa
      121 cagcctggat attcggcagc acaccgcctt gggcaatggt gactttgccc agcagtttat
      181 tcagttcttc gtcgttacgg attgccagct gcaggtgacg cgggataata cgggttttct
      241 tattatcacg agctgcgtta ccagccagtt ccagaatttc tgcggtcagg tattccagaa
      301 cggctgccag atacaccggt gcacctgcac cgacacgttc tgcatagttg cctttgcgca
      361 gcagacgatg aacgcgaccg accggaaatt gcagaccagc acgggaggag cgggttttgg
      421 ctttggcacg ggctttacca ccttgtttac cacgaccaga catggtatat ctccttctta
      481 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg
      541 agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg
      601 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
      661 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
      721 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
      781 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
      841 gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga
      901 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
      961 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
     1021 cacgtttc
//
