LOCUS       sequence-59518-e        7680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Blanchard K, Robic S, Matsumura I
  TITLE     Transformable facultative thermophile Geobacillus stearothermophilus
            NUB3621 as a host strain for metabolic engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2014 May 2.
  PUBMED    24788326
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2014 May 2."
COMMENT     Sequence Label: pIM1708-surT-PsurP-agaN-pNW33N
FEATURES             Location/Qualifiers
     source          1..7680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..42)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(26..42)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(39..61)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    50..66
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(74..80,81..98,99..104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    119..140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(1027..2031)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     primer_bind     complement(2552..2569)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2723..3311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2803..2822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4174..4195
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
       61 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      121 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      181 tgtcgtgcca gcccttcaaa cttcccaaag gcgagcccta gtgacattag aaaaccgact
      241 gtaaaaagta cagtcggcat tatctcatat tataaaagcc agtcattagg cctatctgac
      301 aattcctgaa tagagttcat aaacaatcct gcatgataac catcacaaac agaatgatgt
      361 acctgtaaag atagcggtaa atatattgaa ttacctttat taatgaattt tcctgctgta
      421 ataatgggta gaaggtaatt actattatta ttgatattta agttaaaccc agtaaatgaa
      481 gtccatggaa taatagaaag agaaaaagca ttttcaggta taggtgtttt gggaaacaat
      541 ttccccgaac cattatattt ctctacatca gaaaggtata aatcataaaa ctctttgaag
      601 tcattcttta caggagtcca aataccagag aatgttttag atacaccatc aaaaattgta
      661 taaagtggct ctaacttatc ccaataacct aactctccgt cgctattgta accagttcta
      721 aaagctgtat ttgagtttat cacccttgtc actaagaaaa taaatgcagg gtaaaattta
      781 tatccttctt gttttatgtt tcggtataaa acactaatat caatttctgt ggttatacta
      841 aaagtcgttt gttggttcaa ataatgatta aatatctctt ttctcttcca attgtctaaa
      901 tcaattttat taaagttcat ttgatatgcc tcctaaattt ttatctaaag tgaatttagg
      961 aggcttactt gtctgctttc ttcattagaa tcaatccttt tttaaaagtc aatcccgttt
     1021 gttgaactac tctttaataa aataattttt ccgttcccaa ttccacattg caataataga
     1081 aaatccatct tcatcggctt tttcgtcatc atctgtatga atcaaatcgc cttcttctgt
     1141 gtcatcaagg tttaattttt tatgtatttc ttttaacaaa ccaccatagg agattaacct
     1201 tttacggtgt aaaccttcct ccaaatcaga caaacgtttc aaattctttt cttcatcatc
     1261 ggtcataaaa tccgtatcct ttacaggata ttttgcagtt tcgtcaattg ccgattgtat
     1321 atccgattta tatttatttt tcggtcgaat catttgaact tttacatttg gatcatagtc
     1381 taatttcatt gcctttttcc aaaattgaat ccattgtttt tgattcacgt agttttctgt
     1441 attcttaaaa taagttggtt ccacacatac caatacatgc atgtgctgat tataagaatt
     1501 atctttatta tttattgtca cttccgttgc acgcataaaa ccaacaagat ttttattaat
     1561 ttttttatat tgcatcattc ggcgaaatcc ttgagccata tctgacaaac tcttatttaa
     1621 ttcttcgcca tcataaacat ttttaactgt taatgtgaga aacaaccaac gaactgttgg
     1681 cttttgttta ataacttcag caacaacctt ttgtgactga atgccatgtt tcattgctct
     1741 cctccagttg cacattggac aaagcctgga tttacaaaac cacactcgat acaactttct
     1801 ttcgcctgtt tcacgatttt gtttatactc taatatttca gcacaatctt ttactctttc
     1861 agccttttta aattcaagaa tatgcagaag ttcaaagtaa tcaacattag cgattttctt
     1921 ttctctccat ggtctcactt ttccactttt tgtcttgtcc actaaaaccc ttgatttttc
     1981 atctgaataa atgctactat taggacacat aatattaaaa gaaaccccca tctatttagt
     2041 tatttgtttg gtcacttata actttaacag atggggtttt tctgtgcaac caattttaag
     2101 ggttttcaat actttaaaac acatacatac caacacttca acgcaccttt cagcaactaa
     2161 aataaaaatg acgttatttc tatatgtatc aagaatagaa agaactcgtt tttcgctacg
     2221 ctcaaaacgc aaaaaaagca ctcattcgag tgctttttct tatcgctcca aatcatgcga
     2281 ttttttcctc tttgcttttc tttgctcacg aagttctcga tcacgctgca aaacatcttg
     2341 aagcgaaaaa gtattcttct tttcttccga tcgctcatgc tgacgcacga aaagccctct
     2401 aggcgcatag gaacaactcc taaatgcatg tgaggggttt tctcgtccat gtgaacagtc
     2461 gcatacgcaa tattttgttt cccatactgc attaatgaat cggccaacgc gcggggagag
     2521 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2581 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2641 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2881 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3121 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3301 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3361 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3421 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3481 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3541 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3601 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3661 accagccagc ccgatatggg aaacaaaata ttgcgtatgc gactgttcac atggacgaga
     3721 aaacccctca catgcattta ggagttgttc ctatgcgcct agagggcttt tcgtgcgtca
     3781 gcatgagcga tcggaagaaa agaagaatac tttttcgctt caagatgttt tgcagcgtga
     3841 tcgagaactt cgtgagcaaa gaaaagcaaa gaggaaaaaa tcgcatgatt tggagcgata
     3901 agaaaaagca ctcgaatgag tgcttttttt gcgttttgag cgtagcgaaa aacgagttct
     3961 ttctattctt gatacatata gaaataacgt catttttatt ttagttgctg aaaggtgcgt
     4021 tgaagtgttg gtatgtatgt gattcaataa tttcttttac tcgctcgtta tagtcgatcg
     4081 gttcatcatt caccaaatca taattttcat gtgaccgttc tttatcaata tcgggattcg
     4141 ttttactttc ccgttctctc tgattgtgaa attgaattcg cggccgcttc tagagcatgt
     4201 caaggcgggg accgtgtgaa aaaaggccag cgcttattaa cggtcgattt cgggcttgtc
     4261 caacaaaaag cgaaaagcac gatcaccccg atcattgtga cgaacggcga tgcagtggca
     4321 agtttagaaa aatcatcgga aacaacggcg gtaaaagggg aaacggttct tttccgtata
     4381 gagacgaaag cataatacca tgctgatcgg gggatggcat gatggcatta agaatccaca
     4441 aaattttaaa taagaatgcg gtcgtcgtgt tggatgacgg aaaagaaaaa attgttatgg
     4501 gtgcgggcat cgcgtttcaa aagcgaaaaa atgacattat ccccccggca aaaattgaga
     4561 aaatttttgt tatggaagag gagaacgaaa agtttcaaca attattgcgg acattgccgg
     4621 aagagtatat cgagattgcg gaagagatca tcagttatgc agaaggaaag ctgcaagcgc
     4681 cattaaacaa tcatattcat atcgcgttaa ccgatcactt gtcatttgct attgaacggc
     4741 tcaagcaggg gtatcgcatc caaaataaat tgcttaatga aattaaagtg ctatacaaaa
     4801 cggagtatga aattgggtta tgggccaaac aattgattca agaacggctc ggcattgaaa
     4861 ttccagacga tgaagcggcg catatcgccc ttcatatcca tacggcgaaa atggatgccg
     4921 ccagcatgaa caaaacgctg cggcaaacga cgctcattcg tgaaatgatt gacattatac
     4981 aggcagagtt agacattccc attgacgaag atagtatttc ctaccagcgg ctgcttacgc
     5041 atttgcggtt tgcgatcaat cgtatagaaa acggagagct tctgcactcg atggatgaag
     5101 agatgctggc gctcattcaa acaaagtatg ggaaagaatt tgcctgcgcg cagaagatgg
     5161 cggagcacgc aaaaggcgaa tatggcctag agtttccaga cgcggagctg gcttatattg
     5221 cccttcacat tcaacgttta cgaaagcgtt gacaaactgg gagaaacgaa ttacaatgca
     5281 gataagaaaa caatattgcg ggattgttac tgcacaggca ggcaagacct aaaacgtatg
     5341 aagggaatgg cagattgttt ctgtcatatg ctgccctttg tatgttttag gtctttttgt
     5401 ttttcttcaa aaataaaaaa gagaggggtt cgcccatggc aattgtattt gatccaacaa
     5461 acaaaacctt tcatctacaa gcaaacgata caagctatgt gatgcagctc gttcgatcag
     5521 gatatttatc tcatctttat tggggaaaaa agattagaag tgcaaatgga tcaagaagat
     5581 tccaattttt cgaccgcccg ttctccccta atcccgatcc atccgaccgc acattttctt
     5641 tagatacgct gccgcaagaa tacccggcgt atggcaacac ggattttcgc gcgccggcgt
     5701 atcaagtgca attggaaaac ggggcgacga tctcggattt gcgctataaa acgcatcgca
     5761 tttataaagg aaagccaaag ttaaaagggc tgccggccac ctatgtcgaa cgcgaaaacg
     5821 aggcggaaac attagaaatc gtattggaag accgcgtgat cggattacac gtcacactgc
     5881 tttatacggt gtatgagcgg tggaatgtgg tgacgcgttc tgcccgcttt gaaaaccacg
     5941 gtgcggaacg gatcaagctg ttgcgggcgt taagcatgaa tgtagatttc ccgcatgctg
     6001 actatgaatg gctgcatctc ccgggagcat gggggcggga gcgagcagtg gaaagacggc
     6061 cgcttgtcac cggcatgcag tcggtagaaa gccgtcgcgg cgccagcagc caccagcaaa
     6121 atccatttat cgccttgctg gggaaaaata caaatgaaga tgtcggggaa gtatacggct
     6181 ttagccttgt atacagcggc aatttcctcg ctcaggtcga agtcgatcaa ttccagacaa
     6241 cgcgtgtatc tatgggaata aatccgtttg attttacgtg gctgttagaa ccgggcgagt
     6301 cgtttcagac gccggaagtg gtgatggtgt attccgatca aggattgaat ggaatgtcgc
     6361 aaacttatca tcagctatac cgcacccgcc tagcgcgcgg ggcgttccgc gaccgggagc
     6421 gcccgattct tattaacaac tgggaagcga catattttca ttttaatgaa gaaaaaattt
     6481 tgcgccttgc gaaaacagca gcggaattag ggattgagct atttgtgctc gatgacggat
     6541 ggtttggcaa acgcgatgat gatcacagtt cgttaggaga ttggttcgtc aataaacaga
     6601 agctgccaaa tggactggaa gggctagcaa aaaacattaa ccaaatgggc atgcagtttg
     6661 gtctatgggt agaaccggaa atggtttccg tcgacagcga gctgtatcgg cagcatccgg
     6721 actggtgcct ccatgtgccg aaccgcccgc gctcggaagg aagaaaccag cttgtattag
     6781 actattcccg ccaagaagtg tgcgactata ttattcaagt catttccgat gtgttagcga
     6841 gcgcgccgat ttcgtatgtc aaatgggata tgaaccgcca tatgacggaa atcggctctg
     6901 ccgccctgcc tccggaacgg caacgcgaaa cggctcaccg ctacatgtta gggttgtacc
     6961 gcgtcatgga agaaataaca tcccgttttc cgcatgttct gtttgagagc tgctcgggcg
     7021 gcggaggccg gtttgacccg ggcatgctgt actacatgcc acaaacgtgg acgagtgata
     7081 atacggacgc ggtttcccgc ttaaaaatcc aatacggcac cagccttgtt tatccaatca
     7141 tctcgatggg cgcgcacgtg tcagcggtgc cgaaccacca agttcatcgc attacatcgt
     7201 tggaaatgcg cggtcatgtg gcgatgtcgg gaaactttgg ctatgagctc gatttaacaa
     7261 aactaagtga gaaagaaaaa caaaaagtaa aagaacaagt ggcattttac aaagaaattc
     7321 gccgtcttgt ccaattcgga acgttttatc ggatactcag cccgtttgaa ggcaatgagg
     7381 cggcgtggat gttcgtatcc gaggaccaat cggaagcatt ggttgcctat ttccgcgtgc
     7441 tggcggaagc gaatgcgccg ctgtcgttca tacggttaaa aggactggat ccaaataaag
     7501 attacgaaat tattggcagc ggcgaagtgt atggcggcga tgaactgatg tatgcagggt
     7561 taaatgtgcc acagcgacgc ggcgacttca ttagtgtcat gtggcggttg aaagcagctc
     7621 gttaatggac aaaaagaccg gtgtctagca tgcggctagg cactagtagc ggccgctgca
//
