LOCUS       sequence-59526-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pET-Expotin 1
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggtgccgcg cggcagccat atggctagca tgccagcaat
      121 tatgacaatg ttagcagacc atgcagctcg tcagctgctt gatttcagcc aaaaactgga
      181 tatcaactta ttagataatg tggtgaattg cttataccat ggagagggag cccagcaaag
      241 aatggctcaa gaagtactga cacatttaaa ggagcatcct gatgcttgga caagagtcga
      301 cacaattttg gaattttctc agaatatgaa tacgaaatac tatggactac aaattttgga
      361 aaatgtgata aaaacaaggt ggaagattct tccaaggaac cagtgcgaag gaataaaaaa
      421 atacgttgtt ggcctcatta tcaagacgtc atctgaccca acttgtgtag agaaagaaaa
      481 ggtgtatatc ggaaaattaa atatgatcct tgttcagata ctgaaacaag aatggcccaa
      541 acattggcca acttttatca gtgatattgt tggagcaagt aggaccagcg aaagtctctg
      601 tcaaaataat atggtgattc ttaaactctt gagtgaagaa gtatttgatt tctctagtgg
      661 acagataacc caagtcaaat ctaagcattt aaaagacagc atgtgcaatg aattctcaca
      721 gatatttcaa ctgtgtcagt ttgtaatgga aaattctcaa aatgctccac ttgtacatgc
      781 aaccttggaa acattgctca gatttctgaa ctggattccc ctgggatata tttttgagac
      841 caaattaatc ag
//
