LOCUS       sequence-59528-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pET-Ran(Q69L)
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             780..797
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(850..868)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      864..911
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggct agcatggctg
      121 cgcagggaga gccccaggtc cagttcaaac ttgtattggt tggtgatggt ggtactggaa
      181 aaacgacctt cgtgaaacgt catttgactg gtgaatttga gaagaagtat gtagccacct
      241 tgggtgttga ggttcatccc ctagtgttcc acaccaacag aggacctatt aagttcaatg
      301 tatgggacac agccggcctg gagaaattcg gtggactgag agatggctat tatatccaag
      361 cccagtgtgc catcataatg tttgatgtaa catcgagagt tacttacaag aatgtgccta
      421 actggcatag agatctggta cgagtgtgtg aaaacatccc cattgtgttg tgtggcaaca
      481 aagtggatat taaggacagg aaagtgaagg cgaaatccat tgtcttccac cgaaagaaga
      541 atcttcagta ctacgacatt tctgccaaaa gtaactacaa ctttgaaaag cccttcctct
      601 ggcttgctag gaagctcatt ggagacccta acttggaatt tgttgccatg cctgctctcg
      661 ccccaccaga agttgtcatg gacccagctt tggcagcaca gtatgagcac gacttagagg
      721 ttgctcagac aactgctctc ccggatgagg atgatgacct gtgagcggcc gcactcgagc
      781 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      841 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      901 ggggtttttt gctgaaag
//
