LOCUS       sequence-59532-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pET-GST-TNRC6A-(935-984)
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(230..253)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(260..913)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(280..302)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             complement(926..943)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(953..970)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             989..1011
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctctagtg
       61 cggccgctca tttgtcttgt aacattcctc cagaaaggtc catcttattg ctttgtacat
      121 tttcctctgg tgagtctctc gaaaaactga tgtttgaaaa ctgtttaaca aatggattta
      181 tccacgcttc ttcttgtttg tttccacctt tcatcattcc aagcttcagg ggcccctgga
      241 acagaacttc cagatccgat tttggaggat ggtcgccacc accaaacgtg gcttgccagc
      301 cctgcaaagg ccatgctata tacttgctgg atttcaagta cttatcaatt tgtgggatag
      361 cttcaatacg ttttttaaaa caaactaatt ttgggaacgc atccaggcac attgggtcca
      421 tgtataaaac aacatcaaga gcgtcataca acatgaagtc aggatgggtt acatgatcac
      481 catttaaata tgttttatga cataaacgat cttcgaacat tttcagcatt tcaggtagct
      541 tgctaagaaa atcaactttg agagtttcaa agtctttact atatgcaatt ctcgaaacac
      601 cgtatctaat atccaaaacc gctccttcaa gcattgaaat ctctgcacgc tcttttggac
      661 aaccacccaa catgttgtgc ttgtcagcta tataacgtat gatggccata gactgtgtta
      721 atttaacatc accatcaata taataaggaa gattgggaaa ctccaaaccc aattcaaact
      781 ttttgtttcg ccatttatca ccttcatcgc gctcatacaa atgctcttca tatttttctt
      841 caagatattc caaaagaagt cgagtgggtt gcacaaggcc cttaattttc caataaccta
      901 gtatagggga catgctagcc atatggctgc cgcgcggcac caggccgctg ctgtgatgat
      961 gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa aattatttct
     1021 agaggggaat
//
