LOCUS       sequence-59535-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pSUPER-CXCR4
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        67..281
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     202..221
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(357..373)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(358..374)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(392..808)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(408..424)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(440..462)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
       61 gaattcgaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag tgtcactagg
      121 cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga caggggagtg
      181 gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatg
      241 tctttggatt tgggaatctt ataagttctg tatgagacca cagatcccgt tttcactcca
      301 gctaacattg cttcctgtca caatgttagc tggagtgaaa acttttttaa gcttatcgat
      361 accgtcgacc tcgagagatc gatctaattc cggcgcctag agaaggagtg agggctggat
      421 aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac ggggcggatg
      481 gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga ggggggtcaa
      541 aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca tgcgggccag
      601 gtgaaaagac cttgatctta acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg
      661 gccatccgac gttaaaggtt ggccattctg cagagcagaa ggtaacccaa cgtctcttct
      721 tgacatctac cgactggttg tgagcgatcc gctcgacatc tttccagtga cctaaggtca
      781 aacttaaggg agtggtaaca gtctggccct aattttcaga caaatacaga aacacagtca
      841 gacagagaca acacagaacg atgctgc
//
