LOCUS       sequence-59545-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-(925-934)
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(95..112)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    101..325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      371..799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(458..477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     668..689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(808..828)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gacgagctgt acaagtccgg cggcaagctt ctgagtggca aaaaaaggag aagggaaagg
       61 tgatctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      121 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      181 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      241 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
      301 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
      361 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      421 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      481 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      541 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      601 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      661 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      721 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      781 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
      841 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
      901 gtggaaagtc cccaggctcc ccagcagg
//
