LOCUS       sequence-59559-e        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Mazda M, Nishi K, Naito Y, Ui-Tei K
  TITLE     E-cadherin is transcriptionally activated via suppression of ZEB1 
            transcriptional repressor by small RNA-mediated gene silencing.
  JOURNAL   PLoS One. 2011;6(12):e28688. doi: 10.1371/journal.pone.0028688. Epub
            2011 Dec 21.
  PUBMED    22205962
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28688. doi"; pages: " 
            10.1371/journal.pone.0028688. Epub 2011 Dec 21"
COMMENT     Sequence Label: psiCHECK-215a-2
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    257..305
                     /note="synthetic polyadenylation signal"
     promoter        338..442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             627..1005
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(661..680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg
       61 gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg
      121 aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga
      181 acgagcagta attctaggcg atcgctcgag cccgggaatt caccaagtgc caaccccata
      241 aatggccgct ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
      301 gtgtgaggat ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      361 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      421 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      481 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      541 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      601 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      661 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      721 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      781 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      841 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      901 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      961 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1021 tggcgaacta cttactctag cttcccggca acaattaata ga
//
