LOCUS       sequence-59565-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL016A
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             758..780
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 actttaagaa ggagatatac atatggtgtc caaaggcgaa gaactgttta ccagtgtggt
       61 tccgattctg gtggaactgg atggcgacgt taacggtcat aaatttagtg tgtccggcga
      121 aggtgaaggc gatgcgacct atggcaaact gacgctgaaa ctgctgtgca ccaccggtaa
      181 actgccggtc ccgtggccga ccctggtgac cacgctgggt tatggcgtgc agtgtttcgc
      241 gcgctacccg gaccacatga aacaacacga tttctttaaa agtgccatgc cggaaggcta
      301 tgttcaggaa cgtaccatct ttttcaaaga tgacggtaac tacaaaaccc gcgcggaagt
      361 taaatttgaa ggcgatacgc tggtcaaccg tattgaactg aaaggtatcg acttcaaaga
      421 agatggcaat attctgggtc ataaactgga atataactac aatagccaca acgtgtatat
      481 taccgcggat aaacagaaaa acggcatcaa agccaacttc aaaatccgcc ataacatcga
      541 agacggcggt gttcaactgg ccgatcacta ccagcaaaac accccgattg gtgatggtcc
      601 ggtcctgctg ccggataatc attatctgtc ataccagtcg aaactgttta aagacccgaa
      661 tgaaaaacgt gatcacatgg tgctgctgga atttctgacc gcggccggca ttacggaggg
      721 tatgaacgaa ctgtataaat aagcggatcc gaataatttt gtttaacttt aagaaggaga
      781 tatacatatg gtgagtaaag gcgaggagga caatatggcg atcatcaaag ag
//
