LOCUS       sequence-59567-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL027A
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatggtgagt aaaggcgagg aggacaatat
       61 ggcgatcatc aaagagttca tgcgcttcaa agtccacatg gaaggcagcg ttaatggtca
      121 cgagttcgaa attgagggcg aaggcgaagg tcgtccgtat gagggtacac agaccgctaa
      181 actgaaagtc acgaaaggtg gtccactgcc atttgcttgg gatattctga gcccacagtt
      241 catgtatggc tccaaagcct atgtgaaaca tccggccgat attccggact atctgaaact
      301 gagcttccct gaagggttca aatgggaacg tgtgatgaac tttgaggatg gtggtgttgt
      361 gacagtgaca caggattcta gcctgcaaga cggtgagttc atctataaag tgaaactgcg
      421 tggcacgaat tttccgagtg atggcccggt tatgcagaaa aaaacgatgg gttgggaggc
      481 ctctagtgag cgtatgtatc cagaagatgg cgctctgaaa ggcgaaatca aacagcgtct
      541 gaaactgaaa gatggtggcc actatgatgc cgaagtgaaa accacgtata aagccaaaaa
      601 acctgtccaa ctgcctggtg cctataacgt taacatcaaa ctggacatca cctcacacaa
      661 tgaggactat acgatcgtgg agcagtatga gcgtgctgaa ggacgtcatt ctaccggtgg
      721 tatggatgag ctgtataaat aagcggatcc gaattcaatt agtttgaact tataaggaga
      781 ataatctatg ag
//
