LOCUS       sequence-59573-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-(716-772)
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(237..254)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    243..467
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      513..893
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(600..619)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     810..831
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggacgagctg tacaagtccg gcggcaagct tggctggctg gggggaccta taccagcccc
       61 agcaaaagaa gaagaaccca caggctggga ggaaccatcc ccagaatcta tacgtcgcaa
      121 aatggagatt gatgatggaa cttcagcttg gggagatcca agcaaataca actacaaaaa
      181 tgtgaacatg tggaacaaaa actgatctag agggcccgtt taaacccgct gatcagcctc
      241 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      301 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      361 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
      421 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
      481 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
      541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      841 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
      901 gttaaaaaat gagctgattt aacaaa
//
