LOCUS       sequence-59575-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-(935-1026)-NES-mut
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(348..365)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    354..578
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(711..730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     921..942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggcatggac gagctgtaca agtccggcgg caagcttgga atgatgaaag gtggaaacaa
       61 acaagaagaa gcgtggataa atccagccgt taaacaggct tcaaacgcca gtgcttcgag
      121 agactcacca gaggaaaatg tacaaagcaa taagatggac ctttctggag gaatgttaca
      181 agacaaacga atggagatag ataaacatag cctaaatatt ggtgattaca atcgaacggt
      241 cgggaaaggc cctggttctc ggcctcagat ttccaaagag tcttccatgg agcgcaatcc
      301 ttattttgat aagtgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
      361 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      421 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      481 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      541 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      601 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      721 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      781 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      841 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      901 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaacc
//
