LOCUS       sequence-59577-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-(457-532)
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(304..321)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    310..534
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      580..960
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(667..686)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     877..898
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tctcggcatg gacgagctgt acaagttccg gcggcaagct tagaactgac ttagatccac
       61 gtgtcctgtc caactctggt tggggacaga ctcctattaa gcagaatact gcctgggata
      121 cagaaacatc acctagaggg gaacgaaaga ctgacaatgg gacagaggcc tggggaagct
      181 ctgcaacaca gacttttaac tcaggggcat gtatagataa gactagccct aatggtaatg
      241 atacctcatc tgtatcaggg tggggcgatt gatctagagg gcccgtttaa acccgctgat
      301 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
      361 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
      421 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
      481 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
      541 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
      601 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      661 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      721 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      781 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      841 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      901 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      961 cctattggtt aaaaaatg
//
