LOCUS       sequence-59578-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-(810-924)
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(421..438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    427..651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(784..803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     995..1016
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gcggcaagct tccctggggg gagccttcta
       61 ctccagccac aactgtggat aatggtactt cagcatgggg taagcccata gacagtggtc
      121 ccagctgggg ggaacccatt gctgcggcat ccagcacatc cacgtggggc tccagctctg
      181 ttggtccaca agcattaagc aaatctgggc caaaatctat gcaagatggc tggtgtggtg
      241 atgatatgcc attgcctgga aatcgcccca ctggctggga agaggaagag gatgtggaga
      301 ttggaatgtg gaatagtaat tcatctcaag agcttaactc atctttaaat tggccaccat
      361 atacaaagaa aatgtcatcg aagggttagt ctagagggcc cgtttaaacc cgctgatcag
      421 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      481 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      541 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      601 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      661 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
      721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      841 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      901 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      961 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1021 acactcaacc ctatctcgg
//
