LOCUS       sequence-59595-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
  TITLE     RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
  JOURNAL   Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
  PUBMED    23360965
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508."
COMMENT     Sequence Label: pCas9
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa
       61 gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact
      121 ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga
      181 agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa
      241 ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc
      301 aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc
      361 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
      421 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
      481 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
      541 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
      601 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
      661 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
      721 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
      781 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
      841 tga
//
