LOCUS       sequence-59612-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVtight Blast DEST (w762-1)
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..162
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(187..192,193..210,211..217)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(337..356)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcgactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca aaaaagctga
       61 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      121 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc
      181 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg
      241 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      301 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      361 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      421 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      481 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      541 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
      601 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
      661 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
      721 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgccccc
//
