LOCUS       sequence-59620-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6C-(928-1020)-NES-mut
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(344..361)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    350..574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      620..1000
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(707..726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     917..938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gacgagctgt acaagtccgg cggcaagctt atgaagacgt ctggcaagca ggatgaggcc
       61 tggatcatga gccgggccat caaacaagct acagacgccg gcgctccgag agagccagct
      121 gaggaggcct tgaagagtaa caatatgaat cttgatcagg ccatgagcgc tctgctggaa
      181 aagaaggtgg acgtggacaa gcgtgggctg ggagtgaccg accataatgg aatggccgcc
      241 aagcccctcg gctgccgccc gccaatctcc aaagagtctt ccgtggaccg ccccaccttt
      301 cttgacaagt gatctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
      361 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
      421 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
      481 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
      541 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
      601 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      901 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      961 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1021 ctgatttaac aaaaattta
//
