LOCUS       sequence-59624-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pmyc-GFP-TNRC6A-(925-934)-NLS-mut
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(96..113)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    102..326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      372..800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(459..478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     669..690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(809..829)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggacgagctg tacaagtccg gcggcaagct tctgagtggc aaaaaagccg ctgccgaagc
       61 ttgatctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
      121 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
      181 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
      241 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
      301 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
      361 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      421 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      481 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      541 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      601 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      661 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      721 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      781 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      841 ccaggctccc cagcaggc
//
