LOCUS       sequence-59626-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..112
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             143..256
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccggact ctagcgtttc
       61 caccgcggtg gcggccgcca ccatggatta caaggatgac gacgacgata agtacccata
      121 cgatgttcca gattacgctt ctatggacga gaagaccacc ggctggcggg gcggccacgt
      181 ggtggagggc ctggccggcg agctggagca gctgcgggcc aggctggagc accaccctca
      241 gggccagcgg gagccctgcc cgggcggatc catggatgct gattctgcct ccagttctga
      301 atcagagaga aacatcacta tcatggcttc agggaacaca ggtggtgaaa aagatggcct
      361 tcggaatagc actggacttg gttcccaaaa caagtttgta gttggtagca gcagcaataa
      421 tgtgggccat ggaagtagta ctgggccatg gggtttttcc catggagcca taataagcac
      481 atgtcaggtc tctgtggatg ctcctgaaag caaatctgaa agtagcaaca atagaatgaa
      541 tgcttggggc actgtaagtt cttcatcaaa tggagggtta aatccaagca ctttgaattc
      601 agctagcaac catggtgcct ggccagtatt agagaacaat ggacttgccc taaaagggcc
      661 tgtagggagt ggtagttctg gcattaatat tcagtgcagt actataggcc agatgcctaa
      721 caatcagagt attaactcta aagtgagtgg tggttctacc catggtacct ggggaagcct
      781 tcaggaaact tgtgaatctg aagtaagtgg tacacagaag gtttcattca gtggtcaacc
      841 tcaaaatatt accactgaaa tgactgg
//
