LOCUS       sequence-59630-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..108
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             109..135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             139..252
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag cgtttccacc
       61 gcggtggcgg ccgccaccat ggattacaag gatgacgacg acgataagta cccatacgat
      121 gttccagatt acgcttctat ggacgagaag accaccggct ggcggggcgg ccacgtggtg
      181 gagggcctgg ccggcgagct ggagcagctg cgggccaggc tggagcacca ccctcagggc
      241 cagcgggagc cctgcccggg cggatccatg gatgctgatt ctgcctccag ttctgaatca
      301 gagagaaaca tcactatcat ggcttcaggg aacacaggtg gtgaaaaaga tggccttcgg
      361 aatagcactg gacttggttc ccaaaacaag tttgtagttg gtagcagcag caataatgtg
      421 ggccatggaa gtagtactgg gccatggggt ttttcccatg gagccataat aagcacatgt
      481 caggtctctg tggatgctcc tgaaagcaaa tctgaaagta gcaacaatag aatgaatgct
      541 tggggcactg taagttcttc atcaaatgga gggttaaatc caagcacttt gaattcagct
      601 agcaaccatg gtgcctggcc agtattagag aacaatggac ttgccctaaa agggcctgta
      661 gggagtggta gttctggcat taatattcag tgcagtacta taggccagat gcctaacaat
      721 cagagtatta actctaaagt gagtggtggt tctacccatg gtacctgggg aagccttcag
      781 gaaacttgtg aatctgaagt aagtggtaca cagaaggttt cattcagtgg tcaacctcaa
      841 aatattacca ctgaaatgac tggaccaaat aacactacta actttatgac ctctagttta
      901 ccaaactccg gttcagtgca
//
