LOCUS       sequence-59634-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Mazda M, Nishi K, Naito Y, Ui-Tei K
  TITLE     E-cadherin is transcriptionally activated via suppression of ZEB1 
            transcriptional repressor by small RNA-mediated gene silencing.
  JOURNAL   PLoS One. 2011;6(12):e28688. doi: 10.1371/journal.pone.0028688. Epub
            2011 Dec 21.
  PUBMED    22205962
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28688. doi"; pages: " 
            10.1371/journal.pone.0028688. Epub 2011 Dec 21"
COMMENT     Sequence Label: psiCHECK-302a-1
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    258..306
                     /note="synthetic polyadenylation signal"
     promoter        339..443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             628..1006
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(662..681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg
       61 gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg
      121 aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga
      181 acgagcagta attctaggcg atcgctcgag cccgggaatt cgtattatca tttaaagtgt
      241 attgggccgc tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
      301 tgtgtgagga tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      421 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      481 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      541 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      601 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      661 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      721 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      781 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      841 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      901 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      961 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttg
//
