LOCUS       sequence-59644-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Cha TL, Zhou BP, Xia W, Wu Y, Yang CC, Chen CT, Ping B, Otte AP, 
            Hung MC
  TITLE     Akt-mediated phosphorylation of EZH2 suppresses methylation of 
            lysine 27 in histone H3.
  JOURNAL   Science. 2005 Oct 14;310(5746):306-10.
  PUBMED    16224021
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-10-14"; volume: "310(5746)"; pages: "306-10"
COMMENT     Sequence Label: pcDNA3-3myc-6His-EZH2 21A
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             103..132
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             139..168
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             175..204
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             208..225
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      222..231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 catgagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atccataatc atgggcggcc gcgaacaaaa gttgatttct
      121 gaagaagatt tgaacggtga acaaaagcta atctccgagg aagacttgaa cggtgaacaa
      181 aaattaatct cagaagaaga cttgaaccac catcaccacc accatggcgg ccgcactggg
      241 aagaaatctg agaagggacc agtttgttgg cggaagcgtg taaaagctga gtacatgcga
      301 ctgagacagc tcaagaggtt cagacgagct gatgaagtaa agagtatgtt tagttccaat
      361 cgtcagaaaa ttttggaaag aacggaaatc ttaaaccaag aatggaaaca gcgaaggata
      421 cagcctgtgc acatcctgac ttctgtgagc tcattgcgcg ggactaggga gtgttcggtg
      481 accagtgact tggattttcc aacacaagtc atcccattaa agactctgaa tgcagttgct
      541 tcagtaccca taatgtattc ttggtctccc ctacagcaga attttatggt ggaagatgaa
      601 actgttttac ataacattcc ttatatggga gatgaagttt tagatcagga tggtactttc
      661 attgaagaac taataaaaaa ttatgatggg aaagtacacg gggatagaga atgtgggttt
      721 ataaatgatg aaatttttgt ggagttggtg aatgccc
//
