LOCUS       sequence-59645-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Mazda M, Nishi K, Naito Y, Ui-Tei K
  TITLE     E-cadherin is transcriptionally activated via suppression of ZEB1 
            transcriptional repressor by small RNA-mediated gene silencing.
  JOURNAL   PLoS One. 2011;6(12):e28688. doi: 10.1371/journal.pone.0028688. Epub
            2011 Dec 21.
  PUBMED    22205962
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28688. doi"; pages: " 
            10.1371/journal.pone.0028688. Epub 2011 Dec 21"
COMMENT     Sequence Label: proE-cad178-Luc
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    395..486
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(427..446)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    500..548
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(816..837)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacaggta
       61 ccggaatgcc aagcttccgg gtgcggtcgg gtcgggccgg gctggagcgg gctggagtct
      121 gaactgactt ccgcaagctc acaggtgctt tgcagttccg acgccactga gagggggtgc
      181 gtggctgcag ccaggtgagc cccggaggca ccgccccccg taccgctgat tggctgaggg
      241 ttcacctgcc ggccacagcc aatcagcagc gcggacccct ccccagggcg gagctgacgg
      301 cccgcccacc cggcctcgca tagacgcggt gaccctctag cctggaagat ctcgagcccg
      361 ggctagcacg cgtaagagct cggtacctat cgatagagaa atgttctggc acctgcactt
      421 gcactgggga cagcctattt tgctagtttg ttttgtttcg ttttgttttg atggagagcg
      481 tatgttagta ctatcgattc acacaaaaaa ccaacacaca gatgtaatga aaataaagat
      541 attttattgc ggccgctcca agtacctccc gtaccttaat attacttact tatcatggta
      601 gcttgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      661 gcctgaatgg cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
      721 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
      781 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
      841 taaagaacgt ggactccaac gtcaaagggc
//
