LOCUS       sequence-59647-e         550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG 
            2nd, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson 
            DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC
  TITLE     In vivo genome editing using a high-efficiency TALEN system.
  JOURNAL   Nature. 2012 Nov 1;491(7422):114-8. doi: 10.1038/nature11537. Epub 
            2012 Sep 23.
  PUBMED    23000899
REFERENCE   2  (bases 1 to 550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11537"; journalName: "Nature"; date: "2012-11-1- 1"; 
            volume: "491"; issue: "7422"; pages: "114-8"
COMMENT     Sequence Label: RCIscript-GoldyTALEN-ponzr1R
FEATURES             Location/Qualifiers
     source          1..550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..49
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             50..76
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 aggaggttca acaatggctt cctcccctcc aaagaaaaag agaaaggtta gttggaagga
       61 cgcaagtggt tggtctagag tggatctacg cacgctcggc tacagtcagc agcagcaaga
      121 gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac cacgaggcac tggtgggcca
      181 tgggtttaca cacgcgcaca tcgttgcgct cagccaacac ccggcagcgt tagggaccgt
      241 cgctgtcacg tatcagcaca taatcacggc gttgccagag gcgacacacg aagacatcgt
      301 tggcgtcggc aaacagtggt ccggcgcacg cgccctggag gccttgctca cggatgcggg
      361 ggagttgaga ggtccgccgt tacagttgga cacaggccaa cttgtgaaga ttgcaaaacg
      421 tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc aatgcactga cgggtgcccc
      481 cctgaacctg accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc
      541 gctcgaaacg
//
