LOCUS       sequence-59650-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Mazda M, Nishi K, Naito Y, Ui-Tei K
  TITLE     E-cadherin is transcriptionally activated via suppression of ZEB1 
            transcriptional repressor by small RNA-mediated gene silencing.
  JOURNAL   PLoS One. 2011;6(12):e28688. doi: 10.1371/journal.pone.0028688. Epub
            2011 Dec 21.
  PUBMED    22205962
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28688. doi"; pages: " 
            10.1371/journal.pone.0028688. Epub 2011 Dec 21"
COMMENT     Sequence Label: proE-cad178-Luc-mEbox
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    396..487
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(428..447)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    501..549
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(817..838)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc caagcttccg ggtgcggtcg ggtcgggccg ggctggagcg ggctggagtc
      121 tgaactgact tccgcaagct cacaggtgct ttgcagttcc gacgccactg agagggggtg
      181 cgtggctgca gctaggttag ccccggaggc accgcccccc gtaccgctga ttggctgagg
      241 gtttaccttc cggccacagc caatcagcag cgcggacccc tccccagggc ggagctgacg
      301 gcccgcccac ccggcctcgc atagacgcgg tgaccctcta gcctggaaga tctcgagccc
      361 gggctagcac gcgtaagagc tcggtaccta tcgatagaga aatgttctgg cacctgcact
      421 tgcactgggg acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc
      481 gtatgttagt actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga
      541 tattttattg cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt
      601 agcttgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      661 agcctgaatg gcgaatggca aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
      721 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
      781 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
      841 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
      901 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgc
//
