LOCUS       sequence-59656-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Harada H, Becknell B, Wilm M, Mann M, Huang LJ, Taylor SS, Scott JD,
            Korsmeyer SJ
  TITLE     Phosphorylation and inactivation of BAD by mitochondria-anchored 
            protein kinase A.
  JOURNAL   Mol Cell 1999 Apr;3(4):413-22.
  PUBMED    10230394
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "1999-04"; volume: "3(4)"; pages: "413-22"
COMMENT     Sequence Label: 3603 pGEX4T-1 Bad S112A (104-141)
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(183..200)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(207..860)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(227..249)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(872..894)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    883..899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(907..913,914..929,930..935))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cgaaacgcgc gaggcagatc ggtcagtcag tcacgatgcg gccgctcgag tcgacccggg
       61 aattctcaga gattgggggg agccgaacgc gagcgtcctc gaaaagggct aagctcctcc
      121 tccatccctt catcctcctc ggtccccgct gggtacgcac tgtggcgact ccgagtctcc
      181 atggatccac gcggaaccag atccgatttt ggaggatggt cgccaccacc aaacgtggct
      241 tgccagccct gcaaaggcca tgctatatac ttgctggatt tcaagtactt atcaatttgt
      301 gggatagctt caatacgttt tttaaaacaa actaattttg ggaacgcatc caggcacatt
      361 gggtccatgt ataaaacaac atcaagagcg tcatacaaca tgaagtcagg atgggttaca
      421 tgatcaccat ttaaatatgt tttatgacat aaacgatctt cgaacatttt cagcatttca
      481 ggtagcttgc taagaaaatc aactttgaga gtttcaaagt ctttactata tgcaattctc
      541 gaaacaccgt atctaatatc caaaaccgct ccttcaagca ttgaaatctc tgcacgctct
      601 tttggacaac cacccaacat gttgtgcttg tcagctatat aacgtatgat ggccatagac
      661 tgtgttaatt taacatcacc atcaatataa taaggaagat tgggaaactc caaacccaat
      721 tcaaactttt tgtttcgcca tttatcacct tcatcgcgct catacaaatg ctcttcatat
      781 ttttcttcaa gatattccaa aagaagtcga gtgggttgca caaggccctt aattttccaa
      841 taacctagta taggggacat gaatactgtt tcctgtgtga aattgttatc cgctcacaat
      901 tccacacatt atacgagccg atgattaatt gtcaacagct catttcagaa tatttgccag
      961 aac
//
