LOCUS       sequence-59660-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F
  TITLE     Comprehensive interrogation of natural TALE DNA-binding modules and 
            transcriptional repressor domains.
  JOURNAL   Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
  PUBMED    22828628
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-07-24"; volume: "3:968. doi"; pages: " 
            10.1038/ncomms1962"
COMMENT     Sequence Label: pAAV_TALE-TF(VP64)-BB_V3
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..172)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(222..527)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(324..343)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggaattctta ctcgaggtta atcagcatgt ccaggtcgaa atcatcaagg gcgtcactgc
       61 cgagcatgtc gaggtcaaag tcatcaaggg catccgaacc aagcatgtca aggtcaaaat
      121 catcgagggc gtcacttccc agcatatcca gatcaaaatc gtccaatgcg tcagcccgtc
      181 cgctagctgc cactcgatga ctagtcctct cgggagagac cttatattcc ccagaacatc
      241 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
      301 ataacggata ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
      361 atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc
      421 agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac gtccacaaac
      481 agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc accagcccat
      541 gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc
      601 ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt
      661 gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg
      721 tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat
      781 acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc
      841 tggcttttag taagccagat ctttacgccc cgccctgcca ctcatcgcag tactgttgta
      901 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
      961 cc
//
