LOCUS       sequence-59668-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F
  TITLE     Comprehensive interrogation of natural TALE DNA-binding modules and 
            transcriptional repressor domains.
  JOURNAL   Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
  PUBMED    22828628
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-07-24"; volume: "3:968. doi"; pages: " 
            10.1038/ncomms1962"
COMMENT     Sequence Label: pAAV_TALEN-FokI(WT)-BB_V3
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..607)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     primer_bind     complement(750..769)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctaggaattc ttatgagcgg aaattgatct cgccattgtt aaacttgcgt ctgacttctt
       61 ccagtgtcag agttcccgct ttgatcattt ctccaccaat gagcagttcc tctacggaca
      121 aaaccgcacc attgcagttc gtaatgtgat tcaaccgtgt gagctgggcc ttatagttgc
      181 ctttgaaatg cccagagaca aacaaaaact taaactcggt cactgatgaa ggatacactt
      241 tccaccactc atttggattg atgtgtttgt tcctggtctg attttcttcg acataacgtt
      301 gcatctcatc tgcctgcccg atgggaagat tgtacccacc gctgtaggct ttcgtgtcga
      361 cgatcactcc gtaatcaatc gggctcccca ccgtatagat tgctccgtcg ggttttcgtg
      421 atccaccgag atgcttccct cggtatccat aaaccttcat aaagaactcc attaccttca
      481 tctcaaggat tctgtcttga gtcgagttcc tagcaatttc gataagttcg atgtattcat
      541 gcggtacgta tttcaatttg tgccgcagct ccgacttttt ctcctcaagt tcactcttca
      601 cgagttggga acctgccact cgatgactag tcctctcggg agagacctta tattccccag
      661 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct
      721 tccccgataa cggataccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
      781 tccccgatat gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca
      841 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacgtcc
      901 acaaacagac gataacggct ctctctttta taggtg
//
