LOCUS       sequence-59671-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1124
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(114..341)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(750..773)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttac tcgtattggg cgccagggtg
       61 gtttttcttt tcaccagtga gacgggcaac agccaagctc cggatccgtg ataaccacct
      121 cttagcctta gcacaagatg taaggtggac tccttctgaa tgttgtaatc agacagcgtt
      181 ctaccgtctt ctagctgctt accggcaaag atcaatcttt gttgatctgg agggatacct
      241 tccttgtctt gaattttcga cttaacgttg tcgatggtat cggaagattc aacttccaat
      301 gttatggttt taccggtcaa agtcttgacg aaaatctgca taatctatta gttaaagtaa
      361 agtgggaggg agagttcgag agttcttttc gtattgtagt agatagaaga tatacttatc
      421 cttatccgta cagttcttgc tctttctgaa gtagcacttt cggaagaaga aagataagca
      481 ggcgaattga tccggtaatt tagtgtgtgt atttgtgttt gcgtgtctat agaagtatag
      541 taatttatgc tacaaaggac ctaatgtata aggaaagaat atttagagaa aagaagaaaa
      601 caagagtttt atatacatac agagcacatg catgccatat gatcatgtgt cgtcgcacac
      661 atatatatat gcctgtatgt gtcagcacta aagttgcctg gccatccacg ctatatacac
      721 gcctggcaga tccggggttt tttctccttg acgttaaagt atagaggtat attaacaatt
      781 ttttgttgat acttttatta catttgaata agaagtaata ca
//
