LOCUS       sequence-59672-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1124
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..751)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(81..101)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     696..724
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aatcataaga aattcgctta tttagaagtg gcgcgcccta tttgtatagt tcatccatgc
       61 catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt
      121 tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag
      181 ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt
      241 caatgttgtg tctaattttg aagttagctt tgattccatt cttttgtttg tctgccatga
      301 tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg cccaagaatg tttccatctt
      361 ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga
      421 cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat
      481 aaccttcggg catggcactc ttgaaaaagt catgctgttt catgtgatct gggtatcttg
      541 aaaagcattg aacaccataa gtgagagtag tgacaagtgt tggccatgga acaggtagtt
      601 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct
      661 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga
      721 caactccagt gaaaagttct tctcctttac tcgtattggg cgccagggtg gtttttcttt
      781 tcaccagtga gacgggcaac agccaagctc cggatccgtg ataaccacct cttagcctta
      841 gcacaagatg taaggtggac tccttctgaa tgttgtaatc agacagcgtt ctaccgtctt
      901 ctagctgctt
//
