LOCUS       sequence-59679-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Qiu L, Wang H, Xia X, Zhou H, Xu Z
  TITLE     A construct with fluorescent indicators for conditional expression 
            of miRNA.
  JOURNAL   BMC Biotechnol. 2008 Oct 7;8:77. doi: 10.1186/1472-6750-8-77.
  PUBMED    18840295
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-10-7"; volume: "8:77. doi"; pages: " 
            10.1186/1472-6750-8-77"
COMMENT     Sequence Label: pEGFP-U6
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(100..420)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(161..181)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     polyA_signal    649..696
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     721..740
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ggcggccgcg aaccaatgca ttggccactg caggatatct ctagaggtac caggcctaac
       61 aaagaattca aaaagttaac tgacaggaag tggtgtttaa acaaggcttt tctccaaggg
      121 atatttatag tctcaaaaca cacaattact ttacagttag ggtgagtttc cttttgtgct
      181 gttttttaaa ataataattt agtatttgta tctcttatag aaatccaagc ctatcatgta
      241 aaatgtagct agtattaaaa agaacagaga tctgattatc tgtcttttat cgcacattaa
      301 gcctctatag ttactaggaa atattatatg caaattaacc ggggcagggg agtagccgag
      361 cttctcccac aagtctgtgc gagggggccg gcgcgggcct agagatggcg gcgtcggatc
      421 cactagttct agccaaggct cgagaagtcc cgttgatttt ggtgccaaaa caaactccca
      481 ttgacgtcaa agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac
      541 ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg
      601 gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc
      661 caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta
      721 ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga
      781 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
      841 tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgg
//
