LOCUS       sequence-59683-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt2B-V5
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgccggtttg gtccagccac tctgccttct tgggggcttt gcaagtatgg acgtccacag
      121 tatttctgca ttccttgcac cgtacagcac agcaccagtg gaatttgcac tcacactggg
      181 taacacgggt gactcgagtt gtgtcgtacc ctcggccaca gcacatgatt tcacaaccgt
      241 ctgttccttt tgatgtcttg ctgcagacac ggcctgcagt gcctagggaa cctgcagcct
      301 tgtccaagac acagtaatct ggagagttgt caaagtagac aagatcagtc cgggtggcac
      361 ggcgatagcc ttggcgggct gcggtgaagt tggcaccatc ttgggtggcc atcacctgca
      421 cagccccatc atagcgtcgc cgcaggtaat cacctgtgcg gcggaaatct gagagtgcac
      481 gccagcaggt gcgcagagta caggaaccac tcacgccatg gcacttacac tccagcttca
      541 gaaaccgccg cacagccgtg cgaccacagc ggttattatg taagttcatg agggcccggg
      601 catccttaag cctcttctcc ttggcatcca cgaaggcctt ggcaaaacgg acaccgtagt
      661 ggatgttgtc actgcagcca ccccagtcaa agtccccacg ctggtcatgg tgtcggccac
      721 gggtgtaggg gtcacagctg cacacactca gttcaccctg gctacaggcg cgagtaatag
      781 cgtggactac ccctgctgat gagatggcat atacaaaagc tgcctctcgg ctacttctga
      841 gcatgacacg gccaaagacg gtgtggtccc ggt
//
