LOCUS       sequence-59685-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt3A-V5
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcccttgca ggtgtgcacg tcgtagacgc gcgtgcactc ctggcagctg acgtagcagc
      121 accagtggaa cacgcagcgg cacttctccc ggcgccgctc cgctcgcgcg ttgtggccgc
      181 ggccgcagca cagcaggtcg cagccgtcga tgccgtgcga gctgacgttg caggtgcggt
      241 cgcgcgtgcc gaaggagccc gtctcagggt tgggctcgca gaagttgggc gaggcctcgt
      301 agtagaccag gtcgcgctcc gtgggcacct tgaagtaggt gtagcgcggc cgcagggtct
      361 ccacccagcc gcgggactcc cggtgcttct ccaccaccat ctccgaggcg ctgtcgtact
      421 tgtccttgag gaagtcaccg atggcgcgga agtcgggttg cgaccaccag catgtcttca
      481 cctcgcagct gcccgacagc ccgtggcact tgcacttgag gtgcatgtgg ctggcgatgg
      541 cctggcgccc agcctcgttg ttgtggcggt tcatggctga gcgggcatct ggccggttct
      601 cccgggcgtc ggcgaactcc cgagacacca tcccaccaaa ctcgatgtcc tcgctacagc
      661 caccccactt ccagcccttg cctggtgagc cctggtggcg gctgctgcag ccacagatgg
      721 cggccgtgcc ttctgcacat gagcgtgtca ctgcaaaggc cacaccggct gaggcaatgg
      781 cgtggacaaa ggccgactcc ctggtagctt tgtccagcac gggcccgaag atggccaggc
      841 tgtc
//
