LOCUS       sequence-59687-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt4-V5
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcctcggca cgtgtgcaac tccacgagcc gctggcactg ccggcacttg acgaagcagc
      121 accagtggaa tttgcagctg cagcgttcag ccagctccac ctgcgccgtg tggaagccgc
      181 ggccacagca cagcagctca cagccgtcga tggccttgga cgtcttgttg catgtgcggc
      241 ccctcgtgcc cagcacgccg ctgcgcatgt cctgctcaca gaagtcgggg ctaggctcca
      301 agtacaccag gtcctcatct gtgtgcggct tgaactgtgc gttgcgtggc accagtgccc
      361 tggaggagcc cacgcggcgt ggctccacct cagtggcacc atcaaacttc tccttcagtg
      421 cgtgacccac ctggcggaag ggcggcacgg ctcgccagca cgtctttacc tcacaggagc
      481 ctgacacccc gtggcacttg cattccaccc gcatgtgtgt caggatggcc ttcctgccgg
      541 cctcattgtt gtggaggttc atgagggctc tgctggacga ggcccccttg cttctctccc
      601 gcacatccac aaacgactgt gagaaagcca caccgtaggc gatgttgtca gagcatcctg
      661 accactggaa gccctgtggg ctgaccccat gcactgtcct atcacagccg cacttctcca
      721 gctccccact gctgcacgcc cgcgtcactg caaaggccac acctgccgaa gagatggcgt
      781 acacgaaggc cgcctcccga gtcccttgcg tcaccacctt gccgaagacg ggcaaggagt
      841 cgagtgtgga gcagttccag cgccggttcc ggaactggta ctggcactcc tcaa
//
