LOCUS       sequence-59688-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Nishi K, Nishi A, Nagasawa T, Ui-Tei K
  TITLE     Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated
            gene silencing in the nucleus.
  JOURNAL   RNA. 2013 Jan;19(1):17-35. doi: 10.1261/rna.034769.112. Epub 2012 
            Nov 13.
  PUBMED    23150874
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.034769.112"; journalName: "RNA"; date: "2013-01"; 
            volume: "19"; issue: "1"; pages: "17-35"
COMMENT     Sequence Label: pSUPER-DsRed
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        65..279
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     200..219
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(355..371)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(356..372)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(390..806)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(406..422)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(438..460)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga
       61 attcgaacgc tgacgtcatc aacccgctcc aaggaatcgc gggcccagtg tcactaggcg
      121 ggaacaccca gcgcgcgtgc gccctggcag gaagatggct gtgagggaca ggggagtggc
      181 gccctgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac gtgaaatgtc
      241 tttggatttg ggaatcttat aagttctgta tgagaccaca gatcccgccc cgtaatgcag
      301 aagaagagct tcctgtcact cttcttctgc attacggggc ttttttaagc ttatcgatac
      361 cgtcgacctc gagagatcga tctaattccg gcgcctagag aaggagtgag ggctggataa
      421 agggaggatc gaggcggggt cgaacgagga ggttcaaggg ggagagacgg ggcggatgga
      481 ggaagaggag gcggaggctt agggtgtaca aagggcttga cccagggagg ggggtcaaaa
      541 gccaaggctt cccaggtcac gatgtagggg acctggtctg ggtgtccatg cgggccaggt
      601 gaaaagacct tgatcttaac ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc
      661 catccgacgt taaaggttgg ccattctgca gagcagaagg taacccaacg tctcttcttg
      721 acatctaccg actggttgtg agcgatccgc tcgacatctt tccagtgacc taaggtcaaa
      781 cttaagggag tggtaacagt ctggccctaa ttttcagaca aatacagaaa cacagtcaga
      841 cagagacaac acagaacgat gctgcagcgc tgca
//
