LOCUS       sequence-59693-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt7A-V5
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcccttgca cgtgtacatc tccgtgcgct cgctgcacgt gttgcacttg acatagcagc
      121 accagtggaa cttacagttg cactgccaca cgcgggcgta ctggtgggtg ttgtagccac
      181 gcccacagca catgaggtca cagccgctgg cctggggagc cgtcttgttg caggcgcggc
      241 cctgggtgcc cacactgccg gtcaccgggt cctcctcgca gtagttgggc gacttctcga
      301 tgtacaccag gtccgtgtcc atgggcttgc ggtacgacag tggcttcttg atcttcagga
      361 aggtgggccg cttgttgcgg ctggcacgca caggctccac gtgaacggcc tcgttgtact
      421 tgtccttgag cacgtagccc agctcccgaa actgtggcag tgtggtccag cacgtcttgg
      481 tggtgcacga gcctgacacg ccgtggcact tacattccag cttcatgttc tcctccagga
      541 tctttcggcc tgcctcgttg ttgtgcaagt tcatgagagt ccgggcattc tgcttgatct
      601 cccgggcatc cacaaagacc ttggcgaagc cgatgccgta gcggatgtcg gcagagcagc
      661 caccccactt ccagccctcg tcccggtggt actggccttg cttctctttg tcgcagccac
      721 agtcgctcag gttgccctgg gtacaggcag ctgtgatggc gtgggccacg ccggcggcaa
      781 tgatggcgta ggtgaacgca gcctcccggc tccccacttt gagctccttc ccgaagacgg
      841 tgcgctctcc ca
//
