LOCUS       sequence-59695-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, 
            He X
  TITLE     Disulfide Bond Requirements for Active Wnt Ligands.
  JOURNAL   J Biol Chem. 2014 May 19. pii: jbc.M114.575027.
  PUBMED    24841207
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 19. pii: jbc.M114.575027."
COMMENT     Sequence Label: Active Wnt7B-V5
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctc
       61 cgcccttgca ggtgaagacc tcggtgcgct cgctgcaggt gttgcacttg acgaagcagc
      121 accagtggaa tttgcagttg cactgccaca ccttggtgta ctggtgggtg ttgtagcctc
      181 ggccgcagca catggtgtca cagccgtccg cgccgggcga cgtgcggttg cagagacggc
      241 cctgcgtgcc cacgctgccc gtggccgcgt cctcctcgca gtagttgggc gacttctcaa
      301 tgtacaccag gtctgtctcc atgggcttct gatagctgcg cagctgtttg atgcgcagga
      361 aggtgggctg ccgcagacgg ctggcccgca ccacctccac ctgcacggcc gcgttgtact
      421 tctccttcag caggtggccc acctctcgga acttgggcag cgtggtccag caggttttgg
      481 tggtgcagga gccagacacg ccgtggcact tgcactccag ctgcatccgg tcctctagaa
      541 ccttcctgcc ggcctcattg ttatgcaggt tcatgaggcg ccgcgcgttc ttcttgatct
      601 cccgagcgtc cacgaagcgc cgggagaagt cgatgccgta acgcacgtcg gccgagcagc
      661 cgccccactt ccagccctcg gcttggttgt agtagccctg cttctcgcgg tcgcagccgc
      721 agttgctcag gttcccttgg ctgcaggcag cggtgacggc gtgcgccacg ccagccgcgg
      781 tgatggcgta cgtgaaggca gcctcacggc tccctactcg gagctcttgc ccgaagacgg
      841 tcttctcgcc gagggcagag cagttccagc gtccgaagcg gaactggtac tg
//
